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| Type |
Poster Presentation |
| Area |
Electrochemistry |
| Room No. |
Event Hall |
| Time |
4월 20일 (금요일) 11:00~12:30 |
| Code |
ELEC.P-609 |
| Subject |
Electrochemical aptasensor using Fe3O4-antibody-mediator bioconjugate for thrombin detection |
| Authors |
SAEROMI CHUNG, Yoon Bo Shim*
Department of Chemistry, Pusan National University, Korea
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| Abstract |
The Fe3O4-antibody-mediator bioconjugate was developed for the detection of thrombin using aptasensor in a serum sample. The thrombin was determined by the cathodic currents of a TBO-thrombin antibody modified Fe3O4 at the sensor surface. For this, we applied aptamer as the capture molecule bound to the conducting polymer layer. The bioconjugate and sensor probe was characterized using Voltammetry, transmission electron microscopy (TEM), UV-VIS spectroscopy. The experimental conditions were optimized in terms of pH, binding time, washing time, and applied potential. The dynamic ranges of thrombin were from 1 to 500 nM with detection limit of 0.49 (±0.06) nM. |
| E-mail |
claudia8282@naver.com |
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121st General Meeting of the KCS