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| Type |
Poster Presentation |
| Area |
Life Chemistry |
| Room No. |
Event Hall |
| Time |
4월 20일 (금요일) 11:00~12:30 |
| Code |
BIO.P-284 |
| Subject |
Optical control of cellular morphology with photoswitchable activators of Rho GTPases |
| Authors |
Jeongmin Ju, Hyeyeon Chun, Jihye Seong1,*
Center for diagnosis and treatment of dementia, Korea Institute of Science and Technology, Korea
1Center for Neuromedicine, Korea Institute of Science and Technology, Korea
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| Abstract |
Small Rho GTPases regulate the organization of actin cytoskeleton, playing important roles in cell shape, motility, and neuronal morphogenesis. For example, RhoA mediates actin stress fibers and cell contraction, Rac1 induces membrane ruffles, and Cdc42 produces filopodia. To precisely and locally control Rho GTPases thus control cell motility, we developed an optogenetic tool to modulate the activity of RhoGEF, a positive regulator for Rho GTPases. This photoswitchable RhoGEF (psRhoGEF) is based on reversibly switchable fluorescent protein, Dronpa, which is engineered to change its oligomerization status from dimmer to monomer by light stimulation. We successfully developed psRhoA, psRac1, and psCdc42, by fusing the enzymatic domain of RhoGEF for each Rho GTPase between two Dronpa domains. Our results showed that psRhoGEF, psRacGEF, and psCdc42GEF can selectively activate each Rho GTPase by light, successfully inducing cell contraction, membrane ruffles, and filopodia. These optogenetic actuator can be further applied to precisely and locally control cell motility and neuronal morphogenesis. |
| E-mail |
h16510@kist.re.kr |
|
121st General Meeting of the KCS