121st General Meeting of the KCS

Type Poster Presentation
Area Electrochemistry
Room No. Event Hall
Time 4월 20일 (금요일) 11:00~12:30
Code ELEC.P-616
Subject A Novel Label-free Electrochemical Immunosensors Based on Use of Fragmented Antibody (Fab’) and Electrochemically Active Nanoparticles: Simultaneous Detection of Multiple Biomarkers for Early Diagnosis of Allergic Rhinitis
Authors Sang Gyeong Shin, Jae Ho Shin*, Kihak Gwon, JInsu Kim
Department of Chemistry, Kwangwoon University, Korea
Abstract Among various immunoassay techniques, electrochemical immunosensors have gained considerable interest as a promising approach for rapid, selective and sensitive analysis. Despite many advantages, it is still a challenge to find new approaches that could improve the simplicity, specificity, and sensitivity for clinical application. To enhance performance of antibody-based immunosensors, we introduce a novel approach to design a label-free immunosensors via use of fragmented antibody (i.e., Fab’) and electrochemically active nanoprobes (i.e., ferrocene (Fc)-modified silica nanoparticles). The use of Fab’ may improve the sensor’s sensitivity by the oriented immobilization on the electrode surface. The surface coverage with target proteins specifically bound to Fab’ prohibits the accessibility of electrochemically active nanoprobes to the electrode surface, eventually leading to the revention of the interfacial electron transfer (i.e., blocking effects). To amplify such blocking effects associated with target protein-Fab’ complex formation, negatively charged Fc-modified silica nanoparticles are utilized as a scaffold to design macromolecular electroactive probes. Herein the Fab’ oriented immobilization on a gold surface and properties of Fc-modified silica nanoparticles will be discussed. Furthermore, we investigate the feasibility of the novel label-free immunosensors to simultaneously detect multiple biomarkers (i.e., albumin, Clara cell protein 16, tryptase, and eosiniphil cationic protein) for early diagnosis of allergic rhinitis in nasal discharge. Finally, four biomarkers in artificial nasal discharge are simultaneously determined with multi-array sensor system equipped in a microfluidic device.
E-mail tkdrud0323@naver.com