121st General Meeting of the KCS

Type Poster Presentation
Area Polymer Chemistry
Room No. Event Hall
Time 4월 20일 (금요일) 11:00~12:30
Code POLY.P-40
Subject Preparation, Characterization, and In Vitro Permeability Evaluation of Chitosan Microcapsules as an Oral Delivery Platform for Antisense Therapeutics
Authors Kyungjik Yang, Taehyung Kim, Yoonsuk Lee, Young Hoon Roh*
Biotechnology, Yonsei University, Korea
Abstract Antisense therapeutics have been widely used for treating various diseases as it is able to regulate target protein expressions with high sequence specificity and low toxicity. In addition, oral administration of antisense therapeutics is preferred because it increases patient compliance and cost effectiveness. However, oral delivery of antisense therapeutics is limited due to the inherent instability and low permeability of nucleic acid. To overcome these barriers, we developed chitosan microcapsules for stable and efficient oral delivery of antisense therapeutics. Chitosan has been commonly applied for synthesis of oral delivery platforms owing to its mucoadhesive and tight junction opening properties. Antisense therapeutic nanoparticles were prepared by electrostatic interaction between the nucleic acid and chitosan as a first step of chitosan microcapsules synthesis. The nanoparticles were then entrapped in chitosan microcapsules by ionic gelation between chitosan and phytic acid. The chitosan microcapsules protected antisense therapeutics against environments of gastrointestinal tract such as simulated gastric fluid and nuclease. Furthermore, chitosan microcapsules showed improved in vitro permeability and low cytotoxicity of the antisense therapeutic nanoparticles. The results demonstrate the potential application of chitosan microcapsules as a suitable oral delivery platform for antisense therapeutics.
E-mail kjdominicyang@gmail.com