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|
| Type |
Poster Presentation |
| Area |
Physical Chemistry |
| Room No. |
Event Hall |
| Time |
4월 20일 (금요일) 11:00~12:30 |
| Code |
PHYS.P-196 |
| Subject |
Measurements of complex refractive index change of photoactive yellow protein over a wide wavelength range using hyperspectral quantitative phase imaging |
| Authors |
young min kim, Hyotcherl Ihee1,*
Center for Nanomaterials and Chemical Reactions, Institute for Basic Science, Korea
1Department of Chemistry, Korea Advanced Institute of Science and Technology, Korea
|
| Abstract |
A novel optical holographic technique is presented to simultaneously measure both the real and imaginary components of the complex refractive index (CRI) of a protein solution over a wide visible wavelength range. Quantitative phase imaging was employed to precisely measure the optical field transmitted from a protein solution, from which the CRIs of the protein solution were retrieved using the Fourier light scattering technique. Using this method, we characterized the CRIs of the two dominant structural states of a photoactive yellow protein solution over a broad wavelength range (461–582 nm). The significant CRI deviation between the two structural states was quantified and analysed. The results of both states show the similar overall shape of the expected rRI obtained from the Kramers–Kronig relations. |
| E-mail |
youngmin.kim.k@gmail.com |
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121st General Meeting of the KCS