|
|
| Type |
Poster Presentation |
| Area |
Life Chemistry |
| Room No. |
Event Hall |
| Time |
4월 20일 (금요일) 11:00~12:30 |
| Code |
BIO.P-288 |
| Subject |
Investigation of ECM fibrous protein adsorption onto negative-charged bilayers
|
| Authors |
hyeyoung kim, Kwanwoo Shin*, Minyoung Kim1
Department of Chemistry, Sogang University, Korea
1Chemistry, Sogang University, Korea
|
| Abstract |
In nature, almost all cells make use of a membrane to interconnect the inside and outside environment. Composed of highly asymmetric assemblies, its main structure is based on two sheets of lipid molecules harmonized with a wide variety of biomolecules. This dynamic interface enables membranes to function various things and understanding them is an essential step in devising novel therapies. Many model systems are currently existing to investigate them. In our experiment, supported bilayer (SLB) was used as a basis model and extracellular matrix (ECM) protein adsorption was studied. Especially, in order to observe the effects of negative-charged bilayers on fibrous protein structures, mixtures of DOPC and DOPS lipids, fibronectin, and laminin were used. Herein, we estimate the biological activities of each protein coincident with mechanical changes in bilayers employing a few techniques. X-ray reflectivity technique was used to show vertical structural changes of membrane and infrared spectroscopy was used to reveal the protein adsorption process. Also, each of result was complemented by optical techniques to better understand. |
| E-mail |
hyeyoung91@sogang.ac.kr |
|
121st General Meeting of the KCS