121st General Meeting of the KCS

Type Poster Presentation
Area Material Chemistry
Room No. Event Hall
Time 4월 20일 (금요일) 11:00~12:30
Code MAT.P-484
Subject Long-term Antibacterial Activity of Gentamicin-Bentonite Hybrid Coated Film
Authors Jin Song Jung, Hyoung-Jun Kim1, Jae-Min OH1,*
Department of Chemistry, Yonsei University, Korea
1Chemistry and Department of Medical Chemistry, Yonsei University, Korea
Abstract The antibiotic coating is commonly used for medical products to prevent infection of bacteria. However, antibiotics was easily detached from surface of medical devices. To give proper immobilization, we utilized bentonite (BEN) as drug support. A cationic antibiotic, gentamicin (G) was intercalated into BEN by ion-exchange reaction. X-ray diffraction patterns of antibiotic-nanoparticle hybrid (GB) showed lattice expansion along crystallographic c-axis, suggesting incorporation of antibiotic molecules in interlayer space of BEN. Fourier-transformed infrared spectroscopic results revealed that intact structure of antibiotic molecules was well preserved after intercalation into BEN. From elemental analysis indicated that the amount s of intercalated G were comparable to theoretical maximum loading amount in terms of cation exchange capacity of BEN. According to zeta-potential analysis, surface charge value of BEN and GB was changed from negative to positive charge after hybridization, which indicated surface interaction between BEN and G through electrostatic attraction. Through scanning electron microscope images of BEN and GB, we confirmed that size and morphology of GB were preserved after hybridization. Prepared GB and G were mixed with polyurethane (PU) binder to prepare coating suspension, respectively. Then each suspension was coated on PU film through drop-casting method. To figure out sustainability of G in clay support, PU films coated with GB or G itself were immersed in PBS during 3 and 5 days, respectively. Antibacterial activity was evaluated with Escherichia coli and Bacillus subtilis. The GB coated PU film showed less decrease of antibacterial activity than G coated PU film because BEN acted as drug support to prevent G from releasing.
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