121st General Meeting of the KCS

Type Poster Presentation
Area Organic Chemistry
Room No. Event Hall
Time 4월 19일 (목요일) 11:00~12:30
Code ORGN.P-492
Subject Ratiometric Two-Photon Fluorescence Probe for Quantitative Monitoring of hNQO1 Activity in Colon Tissues
Authors Myoungki Cho, Jae Hyung Jo, Hwan Myung Kim1,*
Department of Energy Systems Research, Ajou University, Korea
1Department of Chemistry / Department of Energy Sys, Ajou University, Korea
Abstract Human NAD(P)H:quinone oxidoreductase 1 (hNQO1) as a flavoenzyme catalyzes two- or four-electron reduction of endogenous and exogenous quinones to their hydroquinone forms.1 Reductions catalyzed by hNQO1 play an important role in cell protecting, detoxification and antioxidant cycle.1 In particular, hNQO1 is over-expressed in tumour cells compared with normal cells of the same origin. Especially human colonic carcinomas show a markedly increase activity of hNQO1.2 In this work, ratiometric two-photon fluorescence probe was designed for quantitative analysis of hNQO1 activity related to human cancer and normal tissues. Using fluorescence microscopy, the hNQO1 activity can be measured without homogenates of the tissue. Moreover, the ratiometric system that change emission wavelength activated with enzyme produces quantitative analysis of hNQO1 activity in different samples through dual channel monitoring.3 Two-photon microscopy employs two near-infrared photons as the excitation source, offers a number of advantages including greater penetration depth (> 500 μm), localization of excitation with minimum background signal, and longer observation times.4 This probe shows perceptible blue-to-yellow emission wavelength change activated with hNQO1, high stability and selectivity. It can real time monitor hNQO1 quantitatively in living cells and human colon tissues.
References
1. Nebert, D. W.; Roe, A. L.; Vandale, S. E.; Bingham, E.; Oakley, G. G. Genet. Med. 2002, 4, 62.
2. Schor, N. A.; Cornelisse, C. J. Cancer Res. 1983, 43, 4850.
3. Lee, H. W.; Heo, C. H.; Sen, D.; Byun, H. O.; Kwak, I. H.; Yoon, G.; Kim, H. M. Anal. Chem. 2014, 86, 10001.
4. Kim, H. M.; Cho, B. R. Chem. Rev. 2015, 115, 5014.
E-mail kimhm@ajou.ac.kr