121st General Meeting of the KCS

Type Poster Presentation
Area Organic Chemistry
Room No. Event Hall
Time 4월 19일 (목요일) 11:00~12:30
Code ORGN.P-498
Subject A ratiomatric two-photon fluorescent probe for γ-glutamyltranspeptidase
Authors Yoon Ji Kim, Hwan Myung Kim1,*
Department of Energy Systems research, Ajou University, Korea
1Department of Chemistry / Department of Energy Sys, Ajou University, Korea
Abstract γ-Glutamyltranspeptidase (GGT) is a cell-surface-bound enzyme, which selectively catalyzes the cleavage of the γ-glutamyl bond in glutathione (GSH)1. It is found that GGT plays important role in cellular GSH and cysteine homeostasis, involving in various physiological and pathological processes2. Indeed, it has been discovered that overexpressed levels of GGT are associated with tumorigenesis in several human cancer cell, including ovarian and colon cancer3. Therefore, GGT has been recognized as a potential biomarker of malignant tumors. From this point of view, there is growing interest in fluorescent probes for GGT and several GGT activatable fluorescent probes have been developed for the detection and imaging of GGT both in tumor cells and living animals4. However, only a few one photon ratiomatric fluorescent probes are known that are favorable for biological imaging. In this work, we developed a new ratiomatric two-photon fluorescent probe for GGT by incorporating the γ-glu-substrate and indocyanine fluorophore. This probe showed both one-and two-photon excited fluorescence and large stokes shift as well as high cell loading ability. The probe was readily activated by GGT and successfully applied for one- and two-photon microscopy imaging in live samples. References 1. D. Yao, D. Jiang, Z. Huang, J. Lu, Q. Tao, Z. Yu and X. Meng. Cancer. 2000, 88, 761 2. A. Fraser, S. Ebrahim, G. D. Smith, D. A. Lawlor, Hepatology. 2007, 46, 158 3. M. H. Hanigan, H. F. Frierson Jr., J. E. Brown, M. A. Lovell, P. T. Taylor, Cancer Res, 1994. 54, 286 4. Y.Urano, M. Sakabe, N. Kosaka, M. Ogawa, M. Mitsunaga, D. Asanuma, M. Kamiya, M. R. Young, T. Nagano, P. L. Choyke, H. Kobayashi, Sci. Transl.Med. 2011, 3, 110
E-mail kimhm@ajou.ac.kr