121st General Meeting of the KCS

Type Poster Presentation
Area Medicinal Chemistry
Room No. Event Hall
Time 4월 19일 (목요일) 11:00~12:30
Code MEDI.P-638
Subject Anti-inflammatory potential of Sparganium erectum L. on LPS-stimulated RAW 264.7 macrophage Cells
Authors Min-Jin Kim, Chang-Hee Kang, Sangmi Yu, CHULHWAN KIM, Sang Chul Jeong*
Freshwater Bioresources Utilization Bureau, Nakdonggang National Institute of Biological Resources, Korea
Abstract The stem of Sparganium erectum L.(SEL), one of the aqatic plants, has been reported to have been used as a medicinal herb, but lacks a scientific basis. Also, It is unknown whether SEL modulate the inflammatory response in RAW 264.7 macrophage cells. The present study was therefore designed to elucidate the pharmacological and biological effects of SEL on the production of pro-inflammatory cytokines and inflammatory mediators in macrophages. The results indicate that SEL is an effective inhibitor of lipopolysaccharide(LPS)-induced nitric oxide(NO) and prostaglandin E2(PGE2) production in RAW 264.7cells. SEL significantly inhibited NO and PGE2 production with an IC50 value of 100 μg/mL and 200 μg/mL. Moreover, SEL could effectively inhibit the LPS-induced production of pro-inflammatory cytokines such as IL-6 and TNF-α in a concentration-dependent manner and decreased to about 48% , 54.6% at a concentration of 200ug/mL. SEL effectively attenuated the expression of inflammation-mediating enzymes, inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2), at the protein level in a concentration-dependent manner. To assess the suitability of SEL for cosmetic applications, we also performed MTT assays on HaCaT keratinocytes. SEL did not display any cytotoxicity in these assays. In conclusion, this study not only provides more evidence that SEL exerts anti-inflammatory activity in macrophage cells, but also sheds light on the potential use of SEL as an attractive candidate for treatment of various inflammation-associate disease
E-mail alswls0428@nnibr.re.kr