121st General Meeting of the KCS

Type Poster Presentation
Area Life Chemistry
Room No. Event Hall
Time 4월 20일 (금요일) 11:00~12:30
Code BIO.P-289
Subject Deposition Multi component ECM and Investigate mechanical properties of ECM vesicles
Authors Minyoung Kim, Nahee Kim1,*, Sungwoo Jeong1,*, huong nguyen thanh1,*, Agustina Setiawati1, Kwanwoo Shin2,*
Chemistry, Sogang University, Korea
1Sogang University, Korea
2Department of Chemistry, Sogang University, Korea
Abstract The extracellular matrix (ECM) has important roles in regulating the development, function and homeostasis of all eukaryotic cells. Essentially, binding of ECM molecules to integrin induces ECM unfolding, thereby spontaneously promoting interactions and fibril assembly. Giant unilamellar vesicle (GUV) is a simple model of complex cells. ECM fiber can be coated on GUV by negative charge. For fibrillogenesis of ECM such as Laminin and Fibronectin outside of vesicle, vesicles were made with PS lipid with negative charge ahead. Then GUV were incubated with ECM dimer at 37℃ during 12hrs and next 12hrs. As a result, Multi component ECM coated vesicle was obtained. We analysed structure of binding ECM and investigate mechanical properties by micropipette aspiration. Fibronectin bind themselves by stretching their length and wholly cover on GUV but Laminin bind together by their arms (not stretching) so not evenly cover on GUV. Multi component of ECM will be a good model of cell because there is no distortion and modulus is similar to real cells. Laminin increase area expansion modulus and this GUV can be reached near by real cell’s modulus of mechanical properties.
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