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| Type |
Poster Presentation |
| Area |
Material Chemistry |
| Room No. |
Event Hall |
| Time |
4월 20일 (금요일) 11:00~12:30 |
| Code |
MAT.P-499 |
| Subject |
Concomitant collection and desalting of neuropeptides on a donut-shaped surface patterns for MALDI mass spectrometry |
| Authors |
Sook Yoon, Chang Young Lee*
Ulsan National Institute of Science and Technology, Korea
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| Abstract |
Signaling peptides have been known to have important role in the physiological mechanisms. Currently available approaches for analyzing peptides have drawbacks such as loss of peptides and complicated steps for sample preparation. We developed an efficient platform for mass analysis of neuropeptides. Drop-drying a droplet of neuropeptides dissolved in artificial seawater on a pattern surface pushed the crystallized salts toward the edge via coffee ring effect as the water evaporated, while irreversibly collecting peptides on the central C18-terminated gold region via hydrophobic interaction. The approach enabled detection of 10 fmol bradykinin by matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS) without additional steps for preconcentration or desalting. The peptides concentrated at the central region was confirmed by mass spectrometry imaging. Letting the droplet evaporate in ambient conditions gave the best results, whereas increasing the adsorption time in a humidified condition significantly decreased the surface coverage of peptides. A simple kinetic model, as well as control experiments, confirmed that such dependence on the evaporation rate was caused by the accelerated desorption of peptides at high ionic strength. The surface patterns can be regenerated multiple times by rinsing off the peptides. Our approach for the one-step collection and desalting of neuropeptides greatly simplifies the sample preparation for MALDI-MS. |
| E-mail |
suk7274@unist.ac.kr |
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121st General Meeting of the KCS