121st General Meeting of the KCS

Type Poster Presentation
Area Organic Chemistry
Room No. Event Hall
Time 4월 19일 (목요일) 11:00~12:30
Code ORGN.P-508
Subject A two-photon ratiometric probe for calcium ions and its application in spinal cord injury
Authors Chang Su Lim, DongJun Lee1, Hwan Myung Kim1,*
Department of Chemistry, Ajou University, Korea
1Department of Energy Systems Research, Ajou University, Korea
Abstract Intracellular Ca2+ ([Ca2+]i) play a role in controlling various cellular functions.1 Abnormal [Ca2+]i is related to human disorders such as neurodegeneration, heart disease, and skeletal muscle defects.2 Most probes for Ca2+ also limit their applications in live sample imaging due to the turn-on response process, rapid photobleaching, and short wavelength excitation light, which can cause photodamage, and have limited tissue imaging depth. A appropriate approach is the use of an emission ratiometric probe with two-photon microscopy (TPM), which employs two near-infrared photons as the excitation source.3 The TPM has become one of the most influential technique for imaging studies in living systems, due to its advantages including greater tissue penetration depth, localization of excitation, low photo-damage, and longer observation times.4 We developed a ratiometric two-photon probe for [Ca2+]i two-photon brightness. Using ratiometric TPM imaging, we can perform the quantitative analysis of [Ca2+]i in live neurons and tissues. This probe derived from dual dyes with different Stokes shifts and probe had dual channel, Ca2+ sensing window and internal reference window, without FRET interference. This ratiometric probe can analyze quantitative [Ca2+]i in live cells and tissues including rat spinal cord tissue.
References
1 Emerit, J.; Edeas, M.; Bricaire, F. Biomed. Pharmacother. 2004, 58, 39.
2 Berchtold, M.W.; Brinkmeier, H.; Münterner, M. Physiol. Rev. 2000, 80, 1215.
3 Helmchen, F.; Denk, W. Nat. Methods 2005, 2, 932.
4 Kim, H.M.; Cho, B. R. Chem. Rev. 2015, 115, 5014.
E-mail kimhm@ajou.ac.kr