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| Type |
Poster Presentation |
| Area |
Analytical Chemistry |
| Room No. |
Event Hall |
| Time |
4월 19일 (목요일) 11:00~12:30 |
| Code |
ANAL.P-283 |
| Subject |
Comparative UPLC-HR-MS/MS based metabolomics and bioactivities analyes of Lycium chinense Miller root |
| Authors |
chun whan choi
Biocenter, Gyeonggido Business and Science Accelerator, Korea |
| Abstract |
Lycium chinense Miller root has been used as a traditional medicine for centuries in Asia because of its positive effects on health. The purpose of this study is to determine the Lycium chinense Miller roots according to cultivation age using ultra-high-resolution mass spectrometry (UHPLC-HR-MS/MS) based metabolomics technique was applied for metabolite profiling of 20 Lycium chinense Miller roots samples aged from 1 to 5 years. Multivariate statistical method such as principal component analysis(PCA) were used to compare the derived patterns among the samples. The data set was subsequently applied to various metabolite selection methods for sophisticated classification with the optimal number of metabolites. The results showed variations in accuracy among the classification methods for the samples of different ages, especially for those aged 3 and 4 years. In addition, we investigated the anti-osteoporotic effects of Lycium chinense Miller root extracts by root ages. These results suggested that Lycium chinense Miller root extracts promote the proliferation, stimulation of osteoblast differentiation and inhibition of osteoclast. This proposed analytical method coupled with multivariate analysis is fast, accurate, and reliable for discriminating the cultivation ages of Lycium chinense Miller root samples and is a potential tool to standardize quality control in the Lycium chinense Miller roots related products. |
| E-mail |
cwchoi78@gmail.com |
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121st General Meeting of the KCS