|
|
| Type |
Poster Presentation |
| Area |
Organic Chemistry |
| Room No. |
Event Hall |
| Time |
4월 19일 (목요일) 11:00~12:30 |
| Code |
ORGN.P-514 |
| Subject |
A Two-photon Fluorescent Probe for Carboxylesterase-2 Activity In Situ |
| Authors |
Sang Jun Park, Ji Su Kang, Hwan Myung Kim1,*
Department of Energy Systems Research, Ajou University, Korea
1Department of Chemistry / Department of Energy Sys, Ajou University, Korea
|
| Abstract |
Carboxylesterase (CEs) is a group of the serine hydrolase that catalyze hydrolytic reactions with various endogenous and exogenous substrates.1 The abnormal activity of CE1 and CE2 is closely related to human diseases. Especailly CE2 has become increasingly interested because the level of CE2 expression is altered when cancer develops.2
Previously, a lot of fluorescent probes have been developed to detect CE2 activity such as fluorescein diacetate (FDA).3 However, these probes have some limitations of imposed by fluorescence turn-on responses using a single detection window, short excitation wavelength, pH-sensitivity and instability in biological environment. An alternative approach for the detection of CE2 in live cell is a two-photon microscopy (TPM) with a ratiometric two-photon probe.
In this work, we have developed a new emission ratiometric TP probes that selectively detect CE2 activity in living sample. These probes can be easily loaded into the cells and shows a remarkable blue-to-yellow emissive color change in response to CE2.
References
1. T. Satoh and M. Hosokawa, Ann. Rev. Pharmacol. Toxicol. 1998, 38, 257–288.
2. G. Xu, W. Zhang, M. K. Ma and H. L. McLeod, Clin. Cancer Res. 2002, 8, 2605–2611.
3. J. Wang, E. T. Williams, J. Bourgea, Y. N. Wong and C. J. Patten, Drug Metab. Dispos. 2011, 39, 1329–1333.
|
| E-mail |
kimhm@ajou.ac.kr |
|
121st General Meeting of the KCS