|
Type |
Poster Presentation |
Area |
Physical Chemistry |
Room No. |
Event Hall |
Time |
4월 20일 (금요일) 11:00~12:30 |
Code |
PHYS.P-217 |
Subject |
Two-color UCNP-STED microscopy by using 1540 nm laser |
Authors |
Kyujin Shin, Eunsang Lee, Kang Taek Lee* Department of Chemistry, Gwangju Institute of Science and Technology, Korea |
Abstract |
Lanthanide-doped upconversion nanoparticle, which is more advantageous than conventional fluorescence probes due to its outstanding photo-stability, enabling bioimaging and single particle tracking without any photo-bleaching or blinking. Although various super-resolution microscopy techniques have been developed, they all suffer from the photo-bleaching or blinking of imaging probes. Recently, upconversion nanoparticle (UCNP) based super-resolution imaging have attracted enormous interest. In this study, we propose a UCNP based STED imaging method by using 1540 nm STED beam. The emission of Yb3+,Er3+ and Yb3+,Tm3+ UCNPs could be modulated by emission depletion of the intermediate state which interacts resonantly with 1540 nm depletion beam. Thus, we can achieve two color STED image in live cell efficiently by employing Yb3+,Er3+ and Yb3+,Tm3+ UCNPs with single STED beam. |
E-mail |
shinkj@gist.ac.kr |
|