|
|
| Type |
Poster Presentation |
| Area |
Analytical Chemistry |
| Room No. |
Event Hall |
| Time |
4월 19일 (목요일) 11:00~12:30 |
| Code |
ANAL.P-286 |
| Subject |
Efficient phosphoproteome analysis of tandem mass tags (TMT) labelled peptides |
| Authors |
Suhwan Kim, Sang-Won Lee*
Department of Chemistry, Korea University, Korea
|
| Abstract |
Protein phosphorylation is one of the most important post-translational modifications of proteins and plays a key role in almost all cellular and developmental processes encompassing signal transduction, translocation and protein-protein interaction. Unfortunately, the analysis of protein phosphorylation is very challenging due to low stoichiometry, thus enrichment process of phosphorylated peptides is necessary prior to analysis by mass spectrometry. There is some report that tandem mass tags (TMT) conjugation interferes with the enrichment process, thus the enrichment must be carried out prior to isobaric labeling. However, this method is time consuming and expensive because each sample has to be enriched separately. In this study, we compared two different methods: one with a column desalting step at mid pH condition to remove TMT reagents before the enrichment process and one without the column desalting step. As a result, we not only identified 11,500 more phosphopeptides in complex pancreatic cancer sample compared to non-desalting method, but also with higher specificity. |
| E-mail |
suhwan_kim@korea.ac.kr |
|
121st General Meeting of the KCS