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|
| Type |
Poster Presentation |
| Area |
Analytical Chemistry |
| Room No. |
Event Hall |
| Time |
4월 19일 (목요일) 11:00~12:30 |
| Code |
ANAL.P-287 |
| Subject |
Comprehensive proteome profiling of human plasma to identify novel biomarker of Alzeheimer’s disease |
| Authors |
Hangyeore Lee, Sang-Won Lee*
Department of Chemistry, Korea University, Korea
|
| Abstract |
Alzheimer’s disease (AD) is the most common dementing disease in the elderly. However, there is no reliable diagnostic tool for AD and mild cognitive impairment (MCI) in a molecular level. In this study, a comprehensive proteome profiling of human plasma was performed to identify novel plasma biomarkers of AD and MCI using LC-MS/MS analysis. A total of 50 plasma samples categorized into five different groups (PIB- normal, PIB+ normal, PIB+ MCI, PIB- MCI and PIB+ AD) were collected. Fourteen most abundant proteins were removed by immunoaffinity depletion. Depleted plasma samples were labeled with the 6-plex TMT reagent for quantitation and extensively fractionated based on mid pH RPLC. After global proteome profiling, N-glyco peptide enrichment was conducted using a modified filter-aided capture and elution (FACE) method. By extensive global proteome profiling and N-glyco proteome profiling, a total of 6,295 protein groups were identified and among those, 357 were determined as differentially expressed proteins (DEPs). |
| E-mail |
han_hgr@hanmail.net |
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121st General Meeting of the KCS