121st General Meeting of the KCS

Type Poster Presentation
Area Analytical Chemistry
Room No. Event Hall
Time 4월 19일 (목요일) 11:00~12:30
Code ANAL.P-293
Subject Unambiguous precursor mass assignment and complementary multi-stage database search increase accuracy and sensitivity in peptide identification of data-independent acquisition mass spectrometry data
Authors Dong-gi Mun, Do-Woon Nam1, Jiwon HONG, Sang-Won Lee*
Department of Chemistry, Korea University, Korea
1Korea University, Korea
Abstract Many proteomic researchers have been turning their interests from obtaining extensive protein identification to providing robust and large-scale quantitative information. Data-independent acquisition (DIA) approach has emerged as a promising strategy with the development of advanced mass spectrometer and data analysis tool. However, accurate and sensitive interpretation of highly complex multiplexed MS/MS spectra acquired through DIA experiment still remains a challenge. In this study, we present a novel DIA data analysis strategy including unambiguous precursor mass assignment and complementary multi-stage database search. Previously developed mPE-MMR (multiplexed post-experimental monoisotopic mass refinement) procedure was introduced to determine precursor mass unambiguously from multiplexed MS/MS spectra acquired through DIA experiment. Generated MS/MS data with accurately assigned precursor mass were subjected to multi-stage database search of MS-GF+, spectral library search and MODplus under narrow precursor ion tolerance. Comparing to conventional DIA data analysis approach which does not consider precursor mass during database search, our strategy effectively increases accuracy and sensitivity in peptide identification.
E-mail cacagong@naver.com