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|
| Type |
Poster Presentation |
| Area |
Life Chemistry |
| Room No. |
Event Hall |
| Time |
4월 20일 (금요일) 11:00~12:30 |
| Code |
BIO.P-298 |
| Subject |
NMR investigation of RPA70N mediated protein-protein interaction
|
| Authors |
Gyuho Yeom, Jinwoo Kim, Dabin Ahn, CHIN-JU PARK*
Department of Chemistry, Gwangju Institute of Science and Technology, Korea
|
| Abstract |
Human DNA helicases, WRN (Werner Syndrome Protein) and FANCJ (Fanconi Anemia Group J) have common interaction partner, Replication Protein A (RPA). Interaction of both helicases with RPA stimulates helicase activities which are crucial in DNA repair processes. Here, we observed interactions of RPA-WRN and RPA-FANCJ by NMR spectroscopy and Fluorescence Polarization anisotropy experiments. The chemical shift perturbations of RPA70N by WRN were more prominent than those by FANCJ. The perturbed regions by WRN and FANCJ were partially overlapped, and located mostly in the basic cleft of RPA70N. Our analysis shows that the common binding strategy underlies for maintaining RPA-helicase interactions. |
| E-mail |
ygh1228@naver.com |
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121st General Meeting of the KCS