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|
| Type |
Poster Presentation |
| Area |
Life Chemistry |
| Room No. |
Event Hall |
| Time |
4월 20일 (금요일) 11:00~12:30 |
| Code |
BIO.P-299 |
| Subject |
Engineering of cell membrane using an amphiphilic polymer |
| Authors |
Jung-Hyun Na, Nam Hyuk Kim, Ju Hee Gwon, Yeon Gyu Yu*
Department of Applied Chemistry, Kookmin University, Korea
|
| Abstract |
Reconstitution of purified membrane proteins into cell surface membrane is critical for the analysis of their molecular functions. Here, we report a simple and efficient method of purified membrane proteins into live cell membrane using an amphiphilic polymer derived from poly-gamma-glutamate, APG. This polymer has detergent-like property which stabilizes a purified human endothelin receptor (ETA) at concentration of 0.01%. Upon mixing ETA stabilized with APG with cultured mammalian cells, ETA was spontaneously inserted into cell surface maintaining cell viability. This method is promising to reconstitute membrane proteins into various types of mammalian cells. |
| E-mail |
mannjh@hanmail.net |
|
121st General Meeting of the KCS