121st General Meeting of the KCS

Type Poster Presentation
Area Life Chemistry
Room No. Event Hall
Time 4월 20일 (금요일) 11:00~12:30
Code BIO.P-300
Subject Versatile antimicrobial coating based on recombinant marine mussel proteins
Authors Su Hyeon Kim, Yong Ho Kim1,*
SAINT, Sungkyunkwan University, Korea
1Department of Chemistry, SKKU Advanced Institute o, Sungkyunkwan University, Korea
Abstract As biomedical engineering became a major field in health care treatment, including diagnosis, monitoring, and therapy, nosocomial (hospital-acquired) infections of implantable medical devices became an important issue that the field should overcome. Contact-killing antimicrobial coatings in which antimicrobial compounds are covalently immobilized to the surface by flexible, hydrophobic polymeric chains to kill the adhered bacteria through disruption of their cell membrane have been developed to solve the problem of release-based coatings. In this study, we developed a novel antimicrobial coating that can be applied to various materials, where the recombinant hybrid protein composed of mussel adhesive protein and antimicrobial peptides is immobilized on the material surfaces and kills the bacteria by contact-killing mechanism. Very thin and durable films composed of the recombinant hybrid protein named hybrid mussel adhesive antimicrobial protein-1 (hybrid MA-1) are dip-coated on target surfaces in very short time to show each function of strong adhesion and antimicrobial activity. The surfaces coated with hybrid MA-1 showed almost 99.9% antimicrobial activity against gram-positive Staphylococcus aureus bacteria, which is common and toxic pathogens agents in healthcare-associated infections. Even after washing more than dozens of times, the coated surfaces maintained the potent antimicrobial activities. This facile and protein-based antimicrobial coating, which is free from cytotoxicity and very robust, holds promise for further development as a powerful technique to prevent medical devices from nosocomial infections
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