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|
| Type |
Poster Presentation |
| Area |
Life Chemistry |
| Room No. |
Event Hall |
| Time |
4월 20일 (금요일) 11:00~12:30 |
| Code |
BIO.P-301 |
| Subject |
Expression and purification of mechanosensive channel protein, tentonin 3 |
| Authors |
Ju Hee Gwon, Jung-Hyun Na, Nam Hyuk Kim, Yeon Gyu Yu*
Department of Applied Chemistry, Kookmin University, Korea
|
| Abstract |
Tentonin-3 is a mechanosensitive channel protein which is activated by mechanical stimuli. To characterize the structure-function relationship of tentonin-3, we established an efficient expression system of a recombinant human tentonin-3 as a fusion protein of P9star in the membrane fraction of E. coli. About 9 mg of P9star-tentonin-3 was expressed in the membrane fraction of Star(DE3) pRARE from 3 L fed-batch culture. The expressed P9star-tentonin-3 was purified using Ni-NTA chromatography and size-exclusion chromatography in homogeneity. The purified protein appears as tetrameric complex and reconstituted into LUVs. The channel opening activity of reconstituted tantonin-3 has been investigated by various types of mechanical stress. |
| E-mail |
wwwj123@kookmin.ac.kr |
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121st General Meeting of the KCS