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|
| Type |
Poster Presentation |
| Area |
Life Chemistry |
| Room No. |
Event Hall |
| Time |
4월 20일 (금요일) 11:00~12:30 |
| Code |
BIO.P-302 |
| Subject |
Interaction of replication protein A with two acidic peptides from human Bloom syndrome protein |
| Authors |
Donguk Kang, Sungjin Lee, CHIN-JU PARK*
Department of Chemistry, Gwangju Institute of Science and Technology, Korea
|
| Abstract |
Bloom syndrome protein (BLM) is one of five human RecQ helicases which maintain genomic stability. Interaction of BLM with replication protein A (RPA) stimulates the DNA unwinding ability of BLM. The interaction is expected to be crucial in DNA damage response systems. Although this stimulation of BLM by RPA is of particular importance in cancer cells, the precise binding surfaces of both proteins are not well understood. In this study, we show by fluorescence polarization anisotropy that both of BLM’s acidic surface peptides specifically bind to RPA70N. Our NMR analysis and docking models show that the basic cleft region of RPA70N is the binding site for both peptides and that the acidic peptide/basic cleft interaction governs RPA-BLM binding. |
| E-mail |
sjlee1055@gmail.com |
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121st General Meeting of the KCS