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|
| Type |
Poster Presentation |
| Area |
Life Chemistry |
| Room No. |
Event Hall |
| Time |
4월 20일 (금요일) 11:00~12:30 |
| Code |
BIO.P-303 |
| Subject |
Expression and purification of human glucagon-like peptide-1 receptor in E. coli, a therapeutic target for type 2 diabetes |
| Authors |
Nam Hyuk Kim, Jung-Hyun Na, Ju Hee Gwon, Yeon Gyu Yu*
Department of Applied Chemistry, Kookmin University, Korea
|
| Abstract |
Glucagon-like peptide-1 (GLP-1) is a major incretin hormone and enhances the release of insulin from pancreatic beta-cells by activating the glucagon-like peptide-1 receptor (GLP-1R) which belong to class B of G protein coupled receptors (GPCRs). To characterize the structure-function relationship of GLP-1R and develop an efficient in vitro assay system, a purified GLP-1R in active conformation is required. In this study, we established an efficient expression system of a recombinant human GLP-1R in the membrane fraction of E. coli. About 50 mg of GLP-1R was purified in homogeneity from 2 L fed-batch culture. The purified GLP-1R stabilized as a soluble and homogeneous form using an amphiphilic polymer derived from poly-gamma-glutamate. The stabilized GLP-1R showed similar physiological activity to native human GLP-1R. The recombinant GLP-1R can be used as a therapeutic target molecule to overcome type 2 diabetes. |
| E-mail |
eve01345@naver.com |
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121st General Meeting of the KCS