121st General Meeting of the KCS

Type Poster Presentation
Area Organic Chemistry
Room No. Event Hall
Time 4월 19일 (목요일) 11:00~12:30
Code ORGN.P-533
Subject A Ratiometric Two-Photon Fluorescent Probe for Hydrogen Polysulfides and its application on a Parkinson’s Disease Model
Authors hyeonjin Choi, Won Tae Kim, Hwan Myung Kim*
Department of Chemistry / Department of Energy Sys, Ajou University, Korea
Abstract Hydrogen polysulfides (H2Sn, n>1) are Hydrogen sulfide (H2S) derived signaling molecules that are produced primary during the cross talk between H2S and reactive oxygen species (ROS)/ reactive nitrogen species (RNS). Earlier, it was believed that H2S is the main signaling molecule in the family of reactive Sulphur species (RSS); however, Evidences suggest that H2Sn might be the actual signaling molecules that activate ion channels, transcription factors, and tumor suppressors with higher potency than H2S.1,2 One example is S-sulfhydration.3 A number of H2Sn molecular markers have been reported using fluorescence spectroscopy to observe H2Sn in living systems in recent decades. However, most of them have limitations imposed by fluorescence turn-on responses and the short excitation wavelengths. An alternative approach for the detection of H2Sn is ratiometric imaging with two-photon microscopy (TPM).4 In this study, we developed a ratiometric TP probe for mitochondrial H2Sn. especially, using thioester carbamate as a H2Sn receptor moiety is greatly selective to H2Sn. using this sensitive selective probe, we observed mitochondrial H2Sn is generated more in PD neuron than in normal neuron. References 1. Y. Kimura, Y. Mikami, K. Osumi, M. Tsugane, J. I. Oka and H. Kimura, FASEB J., 2013, 27, 2451 2. a) T. V. Mishanina, M. Libiad, R. Banerjee, Nat. Chem. Biol. 2015, 11, 457; b) H. Kimura, Proc. Jpn. Acad. Ser. B 2015, 91, 131. 3. H. Kimura, Molecules, 2014, 19, 16146–16157. 4. S. K. Bae, C. H. Heo, D. J. Choi, D. Sen, E. H. Joe, B. R. Cho and H. M. Kim, J. Am. Chem. Soc., 2013, 135, 9915–9923
E-mail kimhm@ajou,ac,kr