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| Type |
Poster Presentation |
| Area |
Medicinal Chemistry |
| Room No. |
Event Hall |
| Time |
4월 19일 (목요일) 11:00~12:30 |
| Code |
MEDI.P-649 |
| Subject |
Beta glucan-bile acid carrier mediated oral antigen delivery targeting M-cell receptors in human intestine triggers mucosal immunity |
| Authors |
Mohammad Nazmul Hasan, Yong-kyu Lee1,*
Green Bio Engineering, Korea National University of Transportation, Korea
1Korea National University of Transportation, Korea
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| Abstract |
Oral vaccines encounter multifarious barriers, including acidic and enzymatic degradation, poor uptake, preventing the immune system to react to inoffensive environmental antigens. We designed an oral antigen delivery system, β-glucan-TCA where β-glucan can encapsulate antigen due to its hollow and porous structure, target β-glucan receptors in M-cells in small intestine and induce mucosal immunity, and TCA (taurocholic acid) helps the oral absorption of the carrier thus accelerate the process. The resultant microparticles were analyzed for their antigen loading efficacy, pH stability, in vitro cytotoxicity and cellular uptake capacity in M-cell model. The findings demonstrate that there is an efficient encapsulation of ovalbumin as a key reference protein for vaccination experiments inside β-glucan-TCA core. The formulation is stable in various pH conditions and nontoxic for living cells. Further investigations of the oral formulation is necessary to confirm the capability of inducing immunity.
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| E-mail |
nazmulchemi@gmail.com |
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121st General Meeting of the KCS