121st General Meeting of the KCS

Type Poster Presentation
Area Life Chemistry
Room No. Event Hall
Time 4월 20일 (금요일) 11:00~12:30
Code BIO.P-306
Subject Ultrasensitive Electrochemical Aptasensor for Latent Tuberculosis-Specific Antigen TB7.7
Authors Jinseong Jeon, Hyungjun Youn1, Jin Her1, JIHYUN MOK1, JONGHOON PARK1, Jae-in So2, sangeon shin1, bareum Kil1, JiHoon Jo1, Ban Changill1,*
Department of Life Sciences, Pohang University of Science and Technology, Korea
1Department of Chemistry, Pohang University of Science and Technology, Korea
2School of Interdisciplinary Bioscience and Bioengineering , Pohang University of Science and Technology, Korea
Abstract Tuberculosis is a common and fatal disease that is caused by infection with Mycobacterium tuberculosis. Patients with latent tuberculosis are estimated at 2 billion people, or one third of the world's population. 10% of them progress to active tuberculosis, and it is important to make an early diagnosis and treatment before they show symptoms. TB7.7, also called Rv2654c, is known to be expressed in Mycobacterium tuberculosis H37Rv, which causes a particularly deadly tuberculosis in humans. In this study, we discovered a novel aptamer specific for TB7.7 and developed the aptamer-based electrochemical sensor using gold electrodes. The aptasensor showed dynamic detection range from 10 fM to 10 nM with a detection limit of 0.6 fM. The aptasensor showed high selectivity for TB7.7, without cross-reacting with other tuberculo-proteins. Furthermore, aptasensor was also able to distinguish sera from patients with tuberculosis infection from healthy human sera. We believe that the aptamer-based electrochemical biosensor developed in this study has a great potential to clinical use in the diagnosis of the latent tuberculosis infection.
E-mail sung0214@postech.ac.kr