|
Type |
Poster Presentation |
Area |
Polymer Chemistry |
Room No. |
Event Hall |
Time |
4월 20일 (금요일) 11:00~12:30 |
Code |
POLY.P-59 |
Subject |
Multi-responsive Injectable Hydrogels of Elastin-Calmodulin Fusion Proteins with Dynamic Properties |
Authors |
Jae Sang Lee, Min Jeong Kang, Dong Woo Lim1,* Department of Bionanotechnology, Hanyang University, Korea 1Department of Bionano Engineering, Hanyang University, Korea |
Abstract |
Stimuli-responsive polymer biomaterials are useful for biomedical applications because they self-assemble into hydrogels under aqueous conditions. Especially, genetically engineered protein-based materials are of growing interest due to their accurate control over molecular design, excellent biocompatibility, and specific biological functions. In this study, the fusion proteins in ABA type triblock form, composed of thermally-responsive elastin-based polypeptide (EBP) and ligand-responsive calmodulin (CalM), were genetically engineered, expressed, and purified using different salts via inverse transition cycling (ITC). They exhibited thermally-triggered gelation in a reversible way under physiological environments. Furthermore, the physically crosslinked hydrogels showed ligand-dependent rheological- and mechanical properties above the transition temperature (Tt) depending on different salts. The injectable hydrogels of multi-responsive fusion protein would be promising for tissue engineering and drug delivery applications. |
E-mail |
ljs01115@hanyang.ac.kr |
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