121st General Meeting of the KCS

Type Poster Presentation
Area Polymer Chemistry
Room No. Event Hall
Time 4월 20일 (금요일) 11:00~12:30
Code POLY.P-59
Subject Multi-responsive Injectable Hydrogels of Elastin-Calmodulin Fusion Proteins with Dynamic Properties
Authors Jae Sang Lee, Min Jeong Kang, Dong Woo Lim1,*
Department of Bionanotechnology, Hanyang University, Korea
1Department of Bionano Engineering, Hanyang University, Korea
Abstract Stimuli-responsive polymer biomaterials are useful for biomedical applications because they self-assemble into hydrogels under aqueous conditions. Especially, genetically engineered protein-based materials are of growing interest due to their accurate control over molecular design, excellent biocompatibility, and specific biological functions. In this study, the fusion proteins in ABA type triblock form, composed of thermally-responsive elastin-based polypeptide (EBP) and ligand-responsive calmodulin (CalM), were genetically engineered, expressed, and purified using different salts via inverse transition cycling (ITC). They exhibited thermally-triggered gelation in a reversible way under physiological environments. Furthermore, the physically crosslinked hydrogels showed ligand-dependent rheological- and mechanical properties above the transition temperature (Tt) depending on different salts. The injectable hydrogels of multi-responsive fusion protein would be promising for tissue engineering and drug delivery applications.
E-mail ljs01115@hanyang.ac.kr