121st General Meeting of the KCS

Type Poster Presentation
Area Polymer Chemistry
Room No. Event Hall
Time 4월 20일 (금요일) 11:00~12:30
Code POLY.P-62
Subject Stimuli-responsive and Surface-adhesive Fusion protein Hydrogels for Biomedical Applications
Authors Jae Hee Lee, Jae Sang Lee, Aamna Basheer, Min Jeong Kang, Dong Woo Lim1,*
Department of Bionanotechnology, Hanyang University, Korea
1Department of Bionano Engineering, Hanyang University, Korea
Abstract Genetically engineered elastin-based polypeptides (EBPs) have been of great interest because they have unique stimuli-responsiveness and biocompatibility. In addition, mussel foot proteins (MFPs) are known as potential bioadhesives because of their unique surface adhesive properties in moisture condition. However, genetically engineered MFPs have limitation of low expression and purification yield, and tyrosine residues of recombinant MFPs are modified to 3, 4-dihydroxyphenylalanine (DOPA) by tyrosine hydroxylation enzyme to exhibit surface adhesion. In this study, we report fusion proteins composed of EBPs and MFPs in tri-block form using bacterial co-expression system to overcome the limitation of MFP expression, purification and modification. Fusion proteins with different block length ratios were biosynthesized by molecular cloning of recursive directional ligation, and expressed with tyrosine hydroxylase for simultaneous hydroxylation of tyrosine residues in E. coli expression system. The fusion proteins were purified by the inverse transition cycling as non-chromatographic protein purification method, and catechol side chains of DOPA were oxidized into quinones by alkaline condition. The fusion proteins were physically crosslinked above the transition temperature of EBPs, forming into hydrogel networks. Moreover, the crosslinked hydrogel exhibited strong adhesion properties under wet condition via covalent- and noncovalent interactions between DOPA and surface molecules. In conclusion, the stimuli-responsive and surface-adhesive fusion protein hydrogels would be potential for biomedical applications.
E-mail jaehee742@hanyang.ac.kr