121st General Meeting of the KCS

Type Poster Presentation
Area Polymer Chemistry
Room No. Event Hall
Time 4월 20일 (금요일) 11:00~12:30
Code POLY.P-63
Subject Genetically Engineered Fusion Protein Nanocages for Nanomedicine Applications
Authors Min Jeong Kang, Jae Sang Lee, Aamna Basheer, Dong Woo Lim1,*
Department of Bionanotechnology, Hanyang University, Korea
1Department of Bionano Engineering, Hanyang University, Korea
Abstract Various protein-based nanostructures for advanced drug delivery systems are of growing interest to treat cancer and other diseases. In this study, we report self-assembled nanocages of genetically engineered fusion proteins, which were composed of penta-helices for self-assembly, elastin-based polypeptide (EBP) with stimuli-responsiveness and receptor targeting peptides to inhibit the cell growth. Fusion protein nanocages were formed by self-assembly of penta-helices with four long helical bundles and short α-helix at two exposure sites. Especially, two different receptor-targeting peptides in the exposure sites and thermally responsive EBP of fusion proteins played major roles as active targeting and non-chromatographic purification tags. The fusion proteins were genetically engineered, expressed and purified by inverse transition cycling. Their thermal-sensitivity and self-assembly were characterized by UV-Vis absorbance, dynamic light scattering, transmission electron microscopy. This work suggests that genetically engineered fusion protein nanocages would be promising for advanced drug delivery systems and diagnostics.
E-mail kmj910322@hanyang.ac.kr