|
Type |
Poster Presentation |
Area |
Polymer Chemistry |
Room No. |
Event Hall |
Time |
4월 20일 (금요일) 11:00~12:30 |
Code |
POLY.P-63 |
Subject |
Genetically Engineered Fusion Protein Nanocages for Nanomedicine Applications |
Authors |
Min Jeong Kang, Jae Sang Lee, Aamna Basheer, Dong Woo Lim1,* Department of Bionanotechnology, Hanyang University, Korea 1Department of Bionano Engineering, Hanyang University, Korea |
Abstract |
Various protein-based nanostructures for advanced drug delivery systems are of growing interest to treat cancer and other diseases. In this study, we report self-assembled nanocages of genetically engineered fusion proteins, which were composed of penta-helices for self-assembly, elastin-based polypeptide (EBP) with stimuli-responsiveness and receptor targeting peptides to inhibit the cell growth. Fusion protein nanocages were formed by self-assembly of penta-helices with four long helical bundles and short α-helix at two exposure sites. Especially, two different receptor-targeting peptides in the exposure sites and thermally responsive EBP of fusion proteins played major roles as active targeting and non-chromatographic purification tags. The fusion proteins were genetically engineered, expressed and purified by inverse transition cycling. Their thermal-sensitivity and self-assembly were characterized by UV-Vis absorbance, dynamic light scattering, transmission electron microscopy. This work suggests that genetically engineered fusion protein nanocages would be promising for advanced drug delivery systems and diagnostics. |
E-mail |
kmj910322@hanyang.ac.kr |
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