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| Type |
Poster Presentation |
| Area |
Medicinal Chemistry |
| Room No. |
Event Hall |
| Time |
4월 19일 (목요일) 11:00~12:30 |
| Code |
MEDI.P-651 |
| Subject |
Direct Monitoring of the Small Molecule-Target Interactions in Cells by Translocation of PKCδ Fusion Proteins
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| Authors |
Namkyoung Kim, Taebo Sim1,*
KU-KIST Graduate School of Converging Science and Technology, Korea University, Korea
1Chemical Kinomics Research Center, Korea Institute of Science and Technology, Korea
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| Abstract |
Target ID by biochemical assay is cost effective and popular method, but it has high false-positive rate.To provide more accurate result, development of cell-based target identification methods is needed.
Chemico-CUPID (Cell-based Un-/identified Protein Interaction Discovery) is cell-based assay that use a bait (target) molecule fused to a protein that changes its localization within the cell following a stimulus.
We identified the candidates as novel target of KIST4547, kinase C and D using chemico-CUPID, and we conducted experiments verifying kinase C is new target of KIST4547 in vitro.
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| E-mail |
nkkim312@kist.re.kr |
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121st General Meeting of the KCS