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|
| Type |
Poster Presentation |
| Area |
Life Chemistry |
| Room No. |
Event Hall |
| Time |
4월 20일 (금요일) 11:00~12:30 |
| Code |
BIO.P-308 |
| Subject |
Small molecule-selective peptides screened from engineered phage library and their application to VOC chemiresistive sensors |
| Authors |
Bo Kyeong Kim, Sang Kyung Kim*
Center for Bio Microsystems, Korea Institute of Science and Technology, Korea
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| Abstract |
Volatile organic compound (VOC) are composed of small molecule that has highly volatile. It can be inhaled into body and chronically might lead to central nerve damage, leukemia and cancer. While a variety of gas sensors are commercially available, VOC sensors with high selectivity is only available for benzene, combining a photo-ionization detector and a filter of benzene-specific polymer. In addition, antibody binding and enzyme reactions, commonly used in selective detection at biosensors, function properly only in limited aqueous conditions. Some peptides illustrated high selectivity comparable to antibody-antigen reaction. Chemical stability of those peptides allows them to work in various environment. We conducted bio-panning using a peptide display phage library. To find candidates with high selectivity, we made a library that has remove their non-recombinant background phage. Small molecule receptors were found by inducing selective binding between peptide and small molecular derivatives. The candidate peptides were validated with binding test in liquid phase and a couple of peptides for ketone and ether group were selected. The selected peptides was integrated into resistance-based sensors such as CNT/Graphene. To proximate attach peptides to CNT/Graphene, the sensing layer was formed using a binding layer of chitosan or nafion. We measured the differential signal between the peptide-sensor and the reference sensor in gas phase. The differential signal for each gas was 2.2% for toluene, 0.8% for methane, and less than 0.3% for benzene and acetone. Also, it was confirmed that the sensors recovered the performance to 88% of the initial measurement when the temperature was applied to 60℃. |
| E-mail |
bk0357@gmail.com |
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121st General Meeting of the KCS