121st General Meeting of the KCS

Type Poster Presentation
Area Life Chemistry
Room No. Event Hall
Time 4월 20일 (금요일) 11:00~12:30
Code BIO.P-310
Subject Engineering, Expression and Structural studies of anti-inflammatory peptide, tIK
Authors hyunjun Jang, Ji Sun Kim, YONGAE KIM*
Department of Chemistry, Hankuk University of Foreign Studies, Korea
Abstract Today, about 1% of the population has rheumatoid arthritis (RA), an acute systemic autoimmune disease that causes chronic inflammation of joints and other body parts. Various immune cells such as T cells, B cells, macrophages and dendritic cells participate in the inflammatory response of RA. Although there are many causes for the disease, it is known that the disease is caused by an imbalance of pro-inflammatory cytokines and anti-inflammatory cytokines. Also, self-antigen recognition through abnormal major histocompatibility complex (MHC) class II-expressing B cells produces antibodies that induce more severe RA. Recent studies have shown that an inhibitor of K562 (IK) cytokine isolated from leukemia cell line inhibits MHC class II expression and that truncated IK (tIK), which lacks the 315-amino acid sequence from the N-terminus, have a similar effect. Therefore, we conducted epitope research to develop tIK as a new anti-inflammatory therapeutic agent for RA patients. First, we analyzed the changes in phosphorylated cell signaling proteins in macrophages isolated from tIK transgenic mice, confirming that tIK and interleukin-10 (IL10) receptor subunit alpha are involved. We were able to identify the epitope (or active site) of tIK involved in anti-inflammatory activity by studying the structure of tIK and IL-10 using homology modeling. Based on these results, we proposed 4 anti-inflammatory peptide candidates and identified the anti-inflammatory activity through the TH17 cell differentiation test. Among them, the 18-mer peptide with anti-inflammatory activity was named tIK-YK4 and the short derivatives 9-mer and 14-mer peptides were also designed. We are trying to identify the relationship between structure of these peptides and anti-inflammatory activity through NMR studies. Currently, we have successfully performed overexpression using E. coli and are optimizing the purification process.
E-mail zizidudgh1@naver.com