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| Type |
Oral Presentation |
| Area |
Oral Presentation for Young Scholars in Physical Chemistry |
| Room No. |
Room 402 |
| Time |
THU 10:48-: |
| Code |
PHYS.O-10 |
| Subject |
Real-time observation of ATP-independent SSB displacement by RecO in Deinococcus Radiodurans |
| Authors |
Jihee Hwang, Seong Keun Kim1,*, Nam Ki Lee1,*
Seoul National University, Korea
1Division of Chemistry, Seoul National University, Korea
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| Abstract |
Deinococcus Radiodurans (DR) is known as a robust bacterium, which displays an outstanding resistance to the radiation and DNA damaging reagents. Here, we investigated the role of drRecO, one of recombination mediated proteins in RecA-loading pathway and essential for the extreme radio-resistance of DR, as a key component in early recombinational process using single-molecule techniques. We observed that drRecO can dissociate drSSB from ssDNA in ATP independent manner, allowing RecA-loading onto drSSB-coated ssDNA. With biochemical analysis and mutagenesis study, we have further elucidated that the reaction mechanism for drSSB displacement by drRecO, which is that drSSB can be dissociated from ssDNA by species-specific RecO though drSSB can bind to ssDNA fairly stronger than drRecO. We suggest that drRecO facilitates the RecA-loading onto drSSB-coated ssDNA by dissociating a small SSB-free space on ssDNA so that DR can survive under extreme environments with a rapid DNA repair system. |
| E-mail |
jihee0448@snu.ac.kr |
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121st General Meeting of the KCS