Quinone moieties are widely distributed in nature and are the key pharmacophore of many clinically important drugs, such as anthracycline, daunorubicin, doxorubicin, mitomycin, mitoanthrone, and saintopin. Among the quinone derivatives, 2,3-disbustituted- 1,4-naphthoquinone show many interesting biological activities such as antitumor, antimalarial, antibacterial etc. Therefore, we have synthesized various 2,3-disubtituted-1,4-naphthoquinone derivatives. We have evaluated the effect of 2,3-heterocyclic-1,4-naphthoquinones on the survival of various cancer cell lines, and furthermore, we have verified the biological activity against anti-cancer immunotherapies.