|
Type |
Poster Presentation |
Area |
Analytical Chemistry |
Room No. |
Event Hall |
Time |
4월 19일 (목요일) 11:00~12:30 |
Code |
ANAL.P-303 |
Subject |
Optimization of method for marker compound analysis of Aster glehni extracts using HPLC |
Authors |
Dongwon Shin, Eunhye Han, Kyungmi Lee, Sangho Lee* Korea Eundan, Korea |
Abstract |
In this study, method validation for content analysis of the extracts of Aster glehni, a native plant of Ulleungdo, was conducted. The operating conditions were composed of LUNA C18 (5 μm, 250 ⅹ 4.6 mm, Phenomenex, Torrance, CA, USA) column, 1.0 mL/min for flow rate, and total 40 minutes for gradient elution parameter. In this process, a problem was posed that marker compound, 3,5-Dicaffeoylquinic acid(3,5-DCQA), peak was not separated completely from adjacent peak. To resolve this problem, resolution improvement of 3,5-DCQA peak was attempted by new analytical method. The resolution improvement research was conducted through changing analytical conditions such as column, flow rate, and gradient elution parameter. A column was changed to Kromasil 100-5-C18 (250 ⅹ 4.6 mm, 5 μm). Flow rate was changed to 0.8 mL/min. Gradient elution parameter was also revised to 26 minutes. A better resolution was confirmed with altered operating conditions. Consequently, method validation was conducted to verify the changed analytical method. The results of changing analytical method, it was obtained that resolution of 3,5-DCQA peak was higher than the standard resolution, 1.5. It was also confirmed that 3,5-DCQA peak was completely separated on the chromatogram without interference by adjacent peak. Furthermore, the results of method validation, system suitability, specificity, linearity, accuracy, and precision were meet the criteria. |
E-mail |
dwshin@eundan.co.kr |
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