121st General Meeting of the KCS

Type Poster Presentation
Area Medicinal Chemistry
Room No. Event Hall
Time 4월 19일 (목요일) 11:00~12:30
Code MEDI.P-657
Subject Downregulation of pro-inflammatory factors by a 70% ethanol extracts of Hemistepta lyrata Bunge (HLB) in lipopolysaccharide-stimulated RAW 264.7 macrophage cells
Authors CHULHWAN KIM, Min-Jin Kim, Sangmi Yu, Sang Chul Jeong, Chang-Hee Kang*
Freshwater Bioresources Utilization Bureau, Nakdonggang National Institute of Biological Resources, Korea
Abstract Hemistepta lyrata Bunge (HLB) has been used for reducing fever and detoxification, dispersing swelling, and eliminating stagnated blood as a folk medicine. However, Anti-inflammatory effect of HLB have not been reported yet. In this study, we identified anti-inflammatory activity of the aerial part of HLB. Inhibition activity of NO production was evaluated in LPS-stimulated RAW 264.7 cells. LPS stimulation significantly increased the NO production, however NO production of HLB extracts (HLBE) in LPS-stimulated RAW 264.7 cell was decreased in a concentration dependent manner. We measured the amount of released PGE2 and IL-6 in LPS-stimulated RAW 264.7 cells using ELISA assay. LPS stimulation significantly increased the amount of PGE2 and IL-6, however HLBE significantly decreased LPS-induced PGE2 and IL-6 production. We found phosphorylation of JNK, ERK and P38 occurred in LPS stimulated RAW 264.7 cells. When HLBE was treated with LPS stimulated RAW 264.7 cells, phosphorylation of ERK and P38 was inhibited and JNK was not affected. Viability of RAW 264.7 cells treated with HLBE was determined using to MTT assay. We indicated viability of RAW 264.7 cells at concentration of HLBE in the absence or presence of LPS. The viability of RAW 264.7 cells was identified 90% or more HLBE did not affect growth of RAW 264.7 cell
E-mail ckdgml3735@nnibr.re.kr