|
Type |
Poster Presentation |
Area |
Material Chemistry |
Room No. |
Event Hall |
Time |
4월 20일 (금요일) 11:00~12:30 |
Code |
MAT.P-547 |
Subject |
Surface charge effect on toxicity of AuNPs; Are cationic AuNPs toxic? |
Authors |
Euiyeon Lee, Youngeun Kwon* Department of Biomedical Engineering (BK21 plus), Dongguk University, Korea |
Abstract |
Gold nanoparticles (AuNPs) hold large potentials for various biomedical applications. AuNPs with various physicochemical properties are reported to have different effects on biological systems but the relationship between the physicochemical properties of AuNPs and their biological effects is not clearly elucidated. It is important to understand and predict the biological effects of modified AuNPs with regard to their physicochemical properties for transition into the clinical setting. Among many variables, here we focused on the surface charges and the structures of ligands. For a systematic approach, we prepared fifteen different mono-dispersed AuNPs based on a sequential ligand exchange method. The prepared AuNPs carried various functional groups derived from amino acids and exhibited a series of surface charges ranging from -42.8±11.8 to +41.8±3.8 mV. Biological assays were performed to gauge the effects of charged AuNPs on various cellular functions and cell viability using mammalian cell lines. The cellular uptake of AuNPs and the subsequent changes in cytoskeletal structures and cell motility were also monitored. These studies showed that the cationic AuNPs showed mixed effects ranging from non-toxic to severely toxic while cytotoxicity of anionic and neutral AuNPs were negligible. The cytotoxic cationic AuNPs inhibited the formation of cytoskeletal structure, DNA replication and, consequently, proliferation of mammalian cells. The oxidative damage on genomic DNA was also observed. We suggested that the toxicity was originated from the ligand structure that can cause lytic. This results suggest that the structure of the ligand, but not the charge of the AuNPs, was an important factor determining the biological effects of AuNPs. |
E-mail |
dmldysl@gmail.com |
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