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|
| Type |
Poster Presentation |
| Area |
Life Chemistry |
| Room No. |
Event Hall |
| Time |
4월 20일 (금요일) 11:00~12:30 |
| Code |
BIO.P-313 |
| Subject |
Dynamics of specific genomic DNA under transcription in living cells |
| Authors |
Sora Yang, JUNG BAE SON1, HyeongJeon An, Nam Ki Lee1,*
Department of Physics, Pohang University of Science and Technology, Korea
1Division of Chemistry, Seoul National University, Korea
|
| Abstract |
A bacterial chromosome is highly condensed and forms an unique structure kind of nucleoid in the center of cell, as the genome is compacted to fit into the small occupied region in a E. coli cell. Transcription is considered as one of the major factors involved in organizing the nucleoid. However, the dynamics of a chromosomal DNA under transcription remained unclear. In this study, we labeled a specific DNA loci with fluorescent fusion proteins and tracked their localization in real time, enabling the direct observation of specific genomic DNA dynamics under transcription in live E.coli cells. We observed the movement of specific gene under transcription toward membrane and the change of DNA dynamics. Our results show that transcription plays a crucial role in subcellular localization of genes and nucleoid organization in E.coli cells. |
| E-mail |
yangsr@postech.ac.kr |
|
121st General Meeting of the KCS