121st General Meeting of the KCS

Type Poster Presentation
Area Life Chemistry
Room No. Event Hall
Time 4월 20일 (금요일) 11:00~12:30
Code BIO.P-314
Subject Neuronal differentiation of pluripotent cells by a sirtuin 1 selective inhibitor
Authors Chang-hee Lee, Injae Shin*
Department of Chemistry, Yonsei University, Korea
Abstract Sirtuin 1 (SIRT1), a class of histone deacetylases, is known to play important roles in neuronal differentiation of pluripotent/multipotent stem cells by regulating expression of neuron-associated genes. Herein, we employed a potent and selective small molecule inhibitor of SIRT1 to determine whether inhibition of SIRT1 activities can lead to the differentiation of P19 cells into neurons. In this effort, P19 cells were treated with EX-527, a selective inhibitor of SIRT1. Neuronal differentiation of the treated cells was examined by immunocytochemical, western blot and RT-PCR analyses. It was found that EX-527 promoted neuronal differentiation of P19 cells without differentiation into astrocytes and cardiac cells. The findings indicate that EX-527 induces selective neurogenesis of P19 cells. Importantly, neurons derived from P19 cells after treatment with EX-527 generated voltage-dependent sodium currents and depolarization-induced action potentials. These findings show that the differentiated neurons have electrophysiological properties. The present study suggests that SIRT1 selective inhibitors could have the potential as chemical inducers to differentiate pluripotent cells into functional neurons.
E-mail leech12311@yonsei.ac.kr