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|
| Type |
Poster Presentation |
| Area |
Life Chemistry |
| Room No. |
Event Hall |
| Time |
4월 20일 (금요일) 11:00~12:30 |
| Code |
BIO.P-316 |
| Subject |
Carbohydrate microarrays for profiling of glycosidase activities |
| Authors |
Hyun jiyoung, Injae Shin*
Department of Chemistry, Yonsei University, Korea
|
| Abstract |
Cellular glycans in the form of glycoconjugates are produced by the action of glycosyltransferases and glycosidases. It is known that genetic deficiencies of lysosomal glycosidases cause lysosomal storage disorders. Also, inhibitors of glycosidases have been developed as therapeutic agents to treat viral infections, diabetes and cancer. Because of the biological and pathological significance of glycosidases, it is of great importance to develop new methods to assess their catalytic activities. In this study glycosidase activities were rapidly assessed using carbohydrate microarrays containing fluorescent probe-conjugated glycans. The microarrays were constructed by immobilizing glycosylated near-infrared (NIR) fluorescent probes containing hydrazide appendages on epoxide-modified glass slides. Several glycosidases were applied to the glycan microarrays and the fluorescence intensities were measured by using a microarray scanner. The results of microarray experiments revealed that carbohydrate microarrays immobilized by glycosylated NIR probes are highly useful for profiling glycosidase activities and determination of IC50 values of glycosidase inhibitors. |
| E-mail |
hyunjy@yonsei.ac.kr |
|
121st General Meeting of the KCS