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|
| Type |
Poster Presentation |
| Area |
Life Chemistry |
| Room No. |
Event Hall |
| Time |
4월 20일 (금요일) 11:00~12:30 |
| Code |
BIO.P-317 |
| Subject |
Simultaneous Investigation of Different Membrane Protein Dynamics in Living Cell Using Multi-Color Single Particle Tracking |
| Authors |
HyeongJeon An, Nam Ki Lee1,*
Department of Physics, Pohang University of Science and Technology, Korea
1Division of Chemistry, Seoul National University, Korea
|
| Abstract |
Recently, super-resolution techniques using photoactivatable fluorescent particle enabled imaging individual molecules distributed closer than classical diffraction limit. Furthermore, tracking each localized particle provides information on dynamics of single molecule, acquiring heterogeneity of motions based on their complex environment in living cell. However, this method to track high density of single particle in living cell, named sptPALM, uses photo-activation which not only limit excitation and emission channel for fluorophore but also is unsuitable for controlling density of fluorescent particles when used for multi-color tracking. Here DNA-PAINT which uses binding and detaching of DNA oligonucleotides to control density of single particle trajectories is adapted to simultaneously track different targets. The DNA strands binds specifically to its complementary strand that enables regulation of each particle density by simply adjusting each concentration of imaging strands in the media. The individual dynamics information of the different protein is gained by single particle tracking simultaneously. |
| E-mail |
ahj0225@postech.ac.kr |
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121st General Meeting of the KCS