121st General Meeting of the KCS

Type Poster Presentation
Area Analytical Chemistry
Room No. Event Hall
Time 4월 19일 (목요일) 11:00~12:30
Code ANAL.P-310
Subject Analysis of Natural Products for Spectral Library by Tandem Mass Spectrometry
Authors Eun Sun Ji, Jong Shin Yoo, Jin Young Kim*
Biomedical Omics Group, Korea Basic Science Institute, Korea
Abstract Natural products are small molecules produced by organisms including primary and secondary metabolites. They include small molecules which have complex chemical structures. Recently, mass spectrometry (MS) has experienced major instrumental advances for natural product with high sensitivity and specificity. However, analysis of natural product is difficult to identify because of complexity of MS results. Therefore, library is necessary for characterization of natural product. We selected 1,400 natural products to consider the diversity of the structure. We analyzed the natural products using various mass spectrometry including Orbitrap MS, Triple Time-of-flight (Triple TOF) MS and Quadrupole Time-of-flight (Q-TOF) MS for spectral library. In this study, all spectra were obtained in positive ion mode and five different collision induced dissociation (CID). The resulting tandem data has built in-house library that included molecular weight (m/z), adducts and collision energy. We suggest that tandem spectral library of natural products is useful information to unknown secondary metabolite analysis for the structural characterization.
E-mail eunsunji@kbsi.re.kr