|
Type |
Poster Presentation |
Area |
Analytical Chemistry |
Room No. |
Event Hall |
Time |
4월 19일 (목요일) 11:00~12:30 |
Code |
ANAL.P-311 |
Subject |
Label free quantitative method using isotope peak intensity of peptide by mass spectrometry |
Authors |
Ki Na Yun, Eun Sun Ji1, Han Bin Oh2, Jong Shin Yoo3, Jin Young Kim3,* Biomedical Omics group, Korea Basic Science Institute, Korea 1Korea Basic Science Institute, Korea 2Department of Chemistry, Sogang University, Korea 3Division of Mass Spectrometry Research, Korea Basic Science Institute, Korea |
Abstract |
Quantitative proteomics measures abundance changes of many proteins among multiple samples in a high-throughput manner. Results from such measurements provide information on how biological systems respond to environmental perturbations at a genomic scale. A number of methods have been developed for quantitative proteomics to obtain high proteome coverage, accurate quantification, and wide applicability to different types of samples. We have developed an automated label-free protein quantification method that utilizes the combined intensity of top three isotope peaks at three highest MS spectral point. It was named three isotopes quantification (TIQ) that allows for a comparative protein analysis in a computational manner. The efficiency of TIQ was demonstrated by a benchmark dataset with two proteomes at known ratios. We accurately detected the mixing ratio over the entire protein expression range, with greater precision for abundant proteins. The performance of TIQ was also compared to that of SWATH-MS in terms of the number of quantified peptides and proteins and the specificity to detect differentially abundant proteins, where SWATH-MS is another approach for label free protein quantification utilizing the MS/MS intensity. There are a few advantages to TIQ. First, because it requires no peak area generation from the extracted ion chromatogram (XIC), it allows high-speed quantitation. Second, it is effective to remove signal interference from co-eluted ions with similar m/z values by evaluating the isotope pattern. And third, considering top three isotope peaks provides more sensitive results with better S/N ratios. |
E-mail |
714kina@kbsi.re.kr |
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