KCS 121st General Meeting

Subject Development of Plasmon Based Imaging Techniques to Break an Optical Diffraction Limit
Code ANAL1-1
Time THU 15 : 40
Area Recent Advances in Analytical Chemistry I: Optical Sensor Platform Based Nanobio Materials
Type Symposium
Authors Kyujung Kim
Department of Optics and Mechatronics Engineering, Pusan National University, Korea
Abstract In this presentation, I introduce plasmon based nanoscale sampling methods to break a diffraction limit with nanostructures in total internal reflection fluorescence (TIRF) microscopy. Various fluorescent molecular targets, such as adenovirus, microtubules and proteins, were used to measure the imaging resolution for a broad application. Significant resolution improvements were achieved down to 70 nm imaging resolution without fundamental deviation from a conventional TIRF microscope. I compared those performances to an extraordinary transmission based axial imaging (EOT-AIM) technique using linear nanoaperture arrays. EOT-AIM uses linear arrays of nanoapertures, each of which samples target fluorescence up to a preset axial distance from surface, in combination with wide-field microscopy for acquisition of lateral images. A current design of nanoapertures provides EOT-AIM with axial super-resolution that is as small as 20 nm for a depth range of 500 nm. Experiments were performed for the measurement of axial distribution of intracellular cholera toxin subunit B molecules within mouse macrophage (RAW264.7) cells. The results were successfully confirmed with conventional confocal and total internal reflection fluorescence microscopy. Eventually, a merged plasmon based super resolution imaging platform was explored to analyze molecular events with nanoscale.