121st General Meeting of the KCS

Type Oral Presentation
Area Oral Presentation of Young Analytical Chemists I
Room No. Room 201A
Time THU 10:31-:
Code ANAL1.O-13
Subject Liquid chromatography/mass spectrometry (LC/MS) analysis of ganglioside analysis: the role of ammonium bicarbonate additive
Authors Jun young Park, Sangwon Cha1,*
Chemistry, Hankuk University of Foreign Studies, Korea
1Department of Chemistry, Hankuk University of Foreign Studies, Korea
Abstract Gangliosides are acidic glycosphingolipids which contain sugar chains with a variety of sialic acid residues. There are hundreds of variations in ganglioside structures based on compositions and structures of oligosaccharide head groups as well as compositions of ceramide cores. However, most LC/MS studies did not include separation of these structural isomers. Recently, we developed a simple and reliable LC method with a conventional C18 column that can effectively resolve ganglioside isomers such as structural isomers of disialogangliosides, designated to GD1a and GD1b, which are known to be major species in mammal brains. In this LC method, the key component was the salt additive, ammonium bicarbonate. Therefore, in this study, we focused on optimizing salt additive condition and trying to reveal a separation mechanism behind this phenomenon. Finally, we applied the LC/MS method with the optimized salt additive condition to the analysis of the total ganglioside extract from porcine brain and successfully analyzed individual isomers of di- and tri- gangliosides.
E-mail pjy931119@naver.com