121st General Meeting of the KCS

Type Oral Presentation
Area Oral Presentation of Young Analytical Chemists II
Room No. Room 201A
Time FRI 10:25-:
Code ANAL2.O-17
Subject Phospholipase A2-responsive UCNPs-loaded Phosphate Micelles for Prostate Cancer Cells Imaging
Authors SHARIPOV MIRKOMIL, Salah Mahmoud Tawfik Ahmed, YONG-ILL LEE*
Department of Chemistry, Changwon National University, Korea
Abstract Rare-earth upconversion nanoparticles (UCNPs) have received the tremendous attention of researchers last decades due to their potential application as biological luminescent labels in bio-imaging application. However, it is still a challenge for researchers to obtain nontoxic, nanoscale, well dispersed and high selective nanoparticles. On the other hand, it has been reported that phospholipase A2(PLA-2) enzyme is abnormally overexpressed in several diseases such as pancreatitis or early stages of prostate cancer. Novel phosphate micelles from biocompatible compounds were synthesized and characterized for selective imaging of prostate cancer cells. Using fluorescence method, CMC value of the synthesized micelles was determined to be 0.638 mM. The activity of phospholipase A2 (PLA-2) enzyme toward the synthesized micelle was investigated and confirmed by LC-MS. This kind of surfactant can form UCNPs-loaded phosphate micelles formed from PLA-2 cleavable surfactant in order to deliver UCNPs directly to prostate tumor cells by reducing side effects on normal biological tissues. TEM results showed that micelles have size ranged from 80 nm to 150 nm; whereas size of UCNPs-loaded micelles ranges from 200nm to 350 nm. Cytotoxicity results confirmed the biocompatibility of micelles. Bio-imaging experiments conducted on KB and HeLa cell lines confirmed encapsulation of UCNPs in micelles thereby reducing the non-selective binding of UCNPs towards cells with low expression of PLA-2. Meanwhile, bio-imaging application on 22Rv1 prostate cancer cell line confirmed the selective release of UCNPs on the surface of the prostate cancer cells.reducing the non-selective binding of UCNPs towards cells with low expression of PLA-2. Meanwhile, bio-imaging application on 22Rv1 prostate cancer cell line confirmed the selective release of UCNPs on the surface of the prostate cancer cells.
E-mail mirkosharipov@gmail.com