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Type |
Oral Presentation |
Area |
Oral Presentation of Young Analytical Chemists II |
Room No. |
Room 201A |
Time |
FRI 10:29-: |
Code |
ANAL2.O-18 |
Subject |
Determination of C18 -ceramide based on paper-based analytical device coupling with mass spectrometry |
Authors |
Shavkatjon Azizov, SHARIPOV MIRKOMIL, Huy B.T., Jae-Min Lim, YONG-ILL LEE* Department of Chemistry, Changwon National University, Korea |
Abstract |
Paper spray ionization has been developed as a direct, fast and low-cost sampling and ionization method for qualitative and quantitative mass spectrometric analysis of C18-ceramide in complex mixtures. Analyte ions are generated by applying a high voltage with a small volume of spray solvent onto a porous substrate. The sample can be preloaded onto paper or mixed into the spray solution.
Wax-printed micro paper-based analytical device (µPAD) coated with paraffin was utilized as a high efficient and low-cost microfluidic device which can be combined in paper spray mass spectrometry (PS-MS). Until now, several methods have been introduced for C18-ceramide analysis. However, µPAD combined with PS-MS method has never used for C18-ceramide analysis. Mostly, high-performance liquid chromatography-mass spectrometry methodology is adapted for the determination of ceramide in human blood and serum samples but it needs sophisticated optimization of instrumentation and sample preparation. Our developed µPAD-paper spray mass spectrometry provides clear advantages over other methods due to reducing the experimental steps and fast operation process for identifying C18-ceramide.
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E-mail |
shavkat9191@gmail.com |
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