121st General Meeting of the KCS

Type Oral Presentation
Area Oral Presentation of Young Analytical Chemists II
Room No. Room 201A
Time FRI 10:29-:
Code ANAL2.O-18
Subject Determination of C18 -ceramide based on paper-based analytical device coupling with mass spectrometry
Authors Shavkatjon Azizov, SHARIPOV MIRKOMIL, Huy B.T., Jae-Min Lim, YONG-ILL LEE*
Department of Chemistry, Changwon National University, Korea
Abstract Paper spray ionization has been developed as a direct, fast and low-cost sampling and ionization method for qualitative and quantitative mass spectrometric analysis of C18-ceramide in complex mixtures. Analyte ions are generated by applying a high voltage with a small volume of spray solvent onto a porous substrate. The sample can be preloaded onto paper or mixed into the spray solution. Wax-printed micro paper-based analytical device (µPAD) coated with paraffin was utilized as a high efficient and low-cost microfluidic device which can be combined in paper spray mass spectrometry (PS-MS). Until now, several methods have been introduced for C18-ceramide analysis. However, µPAD combined with PS-MS method has never used for C18-ceramide analysis. Mostly, high-performance liquid chromatography-mass spectrometry methodology is adapted for the determination of ceramide in human blood and serum samples but it needs sophisticated optimization of instrumentation and sample preparation. Our developed µPAD-paper spray mass spectrometry provides clear advantages over other methods due to reducing the experimental steps and fast operation process for identifying C18-ceramide.
E-mail shavkat9191@gmail.com