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| Type |
Oral Presentation |
| Area |
Oral Presentation for 2018 DOW Chemical Korea Award |
| Room No. |
Room 202A |
| Time |
THU 10:15-: |
| Code |
KCS.O-1 |
| Subject |
A chemical biology route to site-specific authentic protein modifications |
| Authors |
Aerin Yang, Sura Ha, Jihye Ahn, Rira Kim, Sungyoon Kim, Younghoon Lee, Jaehoon Kim, Dieter Söll*, Hee-Yoon Lee*, Hee-Sung Park*
Korea Advanced Institute of Science and Technology, Korea
|
| Abstract |
Many essential biological processes are controlled by posttranslational protein
modifications. The inability to synthetically attain the diversity enabled by these
modifications limits functional studies of many proteins.We designed a three-step
approach for installing authentic posttranslational modifications in recombinant proteins. We first use the established O-phosphoserine (Sep) orthogonal translation system to create a Sep-containing recombinant protein. The Sep residue is then dephosphorylated to dehydroalanine (Dha). Last, conjugate addition of alkyl iodides to Dha, promoted by zinc and copper, enables chemoselective carbon-carbon bond formation. To validate our approach, we produced histone H3, ubiquitin, and green fluorescent protein variants with site-specific modifications, including different methylations of H3K79. The methylated histones stimulate transcription through histone acetylation. This approach offers a powerful tool to engineer diverse designer proteins. |
| E-mail |
red88ar@kaist.ac.kr |
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121st General Meeting of the KCS